The goal of the preclinical work was to investigate the following: to see if low-intensity pulsed ultrasound could enhance the therapeutic efficacy of anti-PD-1 in C57BL/6 mice – bearing intracranial GL261 gliomas; epidermal growth factor receptor variant III (EGFRvIII) specific chimeric antigen receptor (CAR) T cells within NSG mice with established EGFRvIII-U87 gliomas (epidermal growth factor receptor variant III); and a genetically-engineered antigen-presenting cells (APC)-based therapy, producing the T-cell attracting chemokine CXCL10 in the GL261-bearing mice