Principles and Applications of Amplicon Sequencing
Amplicon sequencing is a type of DNA sequencing technique commonly used to analyze a specific genetic region. This technique involves the amplification of a DNA fragment, or amplicon, using techniques such as PCR (polymerase chain reaction) to generate a large number of copies. Once these copies are made, they are sequenced to identify genetic variations or mutations. Amplicon sequencing is often used in research and clinical settings due to its high sensitivity and capability to analyze multiple specific regions simultaneously.